The Lab is dedicated to systems and methodologies for the study of Extracellular Vesicles (EVs).

We work to develop novel technological breakthroughs to accelerate the transition of EV based research to real clinical utility. Current projects are aimed at the implementation of:

  • Multimodal analytical platforms for EV characterization
  • Methods for affinity capturing and purification of EVs

We are among the founders of EVIta: the Italian Society for Extracellular Vesicles

EVs counting and sizing
EVs arrays for biomarkers analysis and discovery
Technologies for EVs isolation and characterization
EVs-based liquid biopsy

Featured publications

Digital detection of biomarkers assisted by nanoparticles: application to diagnostics

Marina Cretich, George G. Daaboul, Laura Sola, M. Selim U¨nlu¨ , and Marcella Chiari

Trends in Biotechnology  2015,  33,  6, 343-350

DOI: 10.1002/anie.201409678

Combined mass quantitation and phenotyping of intact extracellular vesicles by a microarray platform

Paola Gagni, Marina Cretich, LuisaBenussi …. and Marcella Chiari

Analytica Chimica Acta 902 (2016) 160–167

DOI: 10.1016/j.aca.2015.10.017

Loss of exosomes in progranulin-associated frontotemporal dementia

Luisa Benussi , Miriam Ciani, …. Marcella Chiari and Roberta Ghidoni

Neurobiology of Aging 40 (2016) 41-49

DOI: 10.1016/j.neurobiolaging.2016.01.001

Digital Detection of Exosomes by Interferometric Imaging

G. Daaboul, P. Gagni, L. Benussi, P.Bettotti, M. Ciani, M. Cretich, D. Freedman, R. Ghidoni, A. Yalcin Ozkumur, C.Piotto, D. Prosperi, B.Santini, M. Selim Ünlü and M. Chiari

Scientific Reports (2016) 6:  3746

DOI: 10.1039/C8CC06010J

Core technologies and instrumentations

  • Counting and sizing of particles by Nanoparticle Tracking Analysis (NTA) using the Nanosight NS300
  • High-sensitivity fluorescence custom-made microarrays for EVs phenotyping (EV arrays)
  • Label-free EVs counting, sizing an phenotyping by custom-made microarray-based ExoView platform

Projects

INDEX: Integrated nanoparticle isolation and detection system for complete on-chip analysis of exosomes

H2020 FETOPEN — Grant agreement n°766466

http://www.indexproject.eu

HYDROGEX: A Peptide Hydrogel Platform for Extracellular Vesicles Isolation and Multimodal Analysis

Bando Materiali Avanzati

Fondazione Cariplo & Regione Lombardia; Grant n° 2018-1720